Md. Moklesur Rahman Sarker1,2,3
1Department of Pharmacy, Gono University (Bishwabidyalay), Nolam, Mirzanagar, Savar, Dhaka- 1344, Bangladesh; 2Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Tsushima-naka, Kita-ku, Okayama 700-8530, Japan; 3Pharmacology and Toxicology Research Group, Health Med Science Research Network, 3/1 Block F, Lalmatia, Dhaka 1207, Bangladesh
*Correspondence: Prof. Dr. Md. Moklesur Rahman Sarker, Head, Department of Pharmacy, Gono Bishwabidyalay (University), Nolam, Mirzanagar, Savar, Dhaka-1344, Bangladesh; Email: moklesur2002@yahoo.com, moklesur.pharmacy@gonouniversity.edu.bd, dr.moklesur2014@gmail.com; Tel: +8801776758882
Although Ayurved medicines have 3000 years long history of traditional use for the treatment of diseases, scientific evidences in support of the therapeutic effect and safety of most of the preparations yet to be explored and justified. The present study aimed to evaluation of the immunostimulating activity of five preparations of Ayurved medicines namely Kanakasav, Dasamularista, Draksharista, Saribadi and Bramhi Rasayan manufactured and marketed in Bangladesh. Freshly prepared Balb/c mice splenocytes were treated with 1, 5 and 10% (v/v) of different Ayurved preparations and the cells were sub-cultured at 37ºC, humidified atmosphere containing 5% CO2 for 120 hours. The production of IgM and IgG antibodies, and proliferation of cells were determined by enzyme-linked immunosorbent assay (ELISA), and 3-(4,5-dimethylthiazol-2-y)-2,5-diphenylterazolium bromide (MTT) methods, respectively. Among the five preparations tested, Kanakasava and Saribadi stimulated the proliferation of splenocytes and enhanced production of both IgM and IgG antibodies. Interestingly, KNK at the dose of 1% exerted immunostimulating activities but the higher doses 5% and 10% did not show any stimulating activities, rather those concentrations of KNK were found to be toxic to the spleen cells. Similarly, 1% concentration of SDI stimulated the splenocytes proliferation and IgM and IgG antibodies productions. Higher doses resulted with the decreased production of IgM and IgG antibodies by the 5% and 10% concentrations of SDI because of the similar reason as illustrated for KNK. This result is also an indicator of narrow safety profile of both of these Ayurved medicines, KNK and SDI. The other three Ayurved preprations - Dasamularista, Draksharista and Bramhi Rasayan failed to induce immunostimulation in vitro. Although Bramhi Rasayan (BRS) showed stimulating activity at the dose of 10% (v/v), it could not induce the production of antibody. This is the first report on the evaluation of immunostimulating activities of Kanakasav, Dasamularista, Draksharista, Saribadi and Bramhi Rasayan which resulted the preliminary immunostimulating activities of Kanakasav and Saribadi. Further investigations are required with the comprehensive experimental studies to explore its immunostimulating potential and safety of the two preparations.
Keywords: Ayurvedic medicine, Kanakasav, Dasamularista, Draksharista, Saribadi, Bramhi Rasayan, Immunostimulant, Splenocytes, IgM, IgG, Differentiation, Proliferation, BALB/c mice.
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